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He said that the recent case hearing in the IHC also showed that the government has repeatedly been under the influence of the tobacco industry, leading public health advocates and the anti-tobacco lobby to worry this will happen again if concrete measures are not taken
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For protein isolation, cells were washed with cold PBS, collected in lysis buffer using a cell scraper, incubated on ice for 20 min, and centrifuged for 5 min at 500 rpm
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